双相体系中固定化酶水解黄芩苷制备黄芩素的研究(4)
[Abstract] The optimum conditions of baicalin hydrolysis into baicalein by immobilized β-glucosidase in a two-phase system was studied and the yield was observed. A two-phase system comprising of sodium acetate buffer and chloroform was determined by comparing the solubleness of baicalein in different solvents and partition coefficient of baicalein in related aqueous-organic two-phase system. β-Glucosidase was immobilized by the crosslinking-embedding method using sodium alginate as the carrier. The optimum reaction temperature， pH value， Michaelis constant， the thermal stability and pH stability were assayed. By comparing the yield of baicalin hydrolysis into baicalein by immobilized β-glucosidase in two-phase system， the optimum reaction conditions were determined-the optimum reaction temperature， pH value and time were 50 ℃， 5.0 and 10 h，respectively. The yield of baicalein was 85.28%. Compare with one-phase system， two-phase system had an advantage in reaction rate and yield.
[Key words] immobilized β-glucosidase； baicalin； baicalein； two-phase system； biocatalysis
doi：10.4268/cjcmm20140423, http://www.100md.com(杨轶舜 程涛 杨骏 张彤 蔡贞贞)
[Key words] immobilized β-glucosidase； baicalin； baicalein； two-phase system； biocatalysis
doi：10.4268/cjcmm20140423, http://www.100md.com(杨轶舜 程涛 杨骏 张彤 蔡贞贞)